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Non-Mendelian inheritance of macronuclear mutations is gene specific in Paramecium tetraurelia.

机译:在四草履虫中大分子突变的非孟德尔遗传是基因特异性的。

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摘要

Paramecium tetraurelia contains two types of nuclei, a diploid germinal micronucleus and a large transcriptionally active macronucleus. The macronuclear genome is formed from the micronuclear DNA during sexual reproduction. Previous studies have shown that the processing of the A-type variable surface protein gene during formation of a new macronucleus is dependent on the presence of the A gene in the old macronucleus. It is not clear if this is a general feature that controls the formation of the Paramecium macronuclear genome or a unique feature of the A locus. Using micronuclear transplantation, we have constructed a strain that has a wild-type micronucleus but has macronuclear deletions of the A- and B-type surface protein genes. Neither the A nor the B gene is incorporated into the new macronucleus after sexual reproduction. Macronuclear transformation of this strain with the B gene rescues the B-gene deletion after formation of the next macronucleus but has not effect on the A deletion. Similarly, transformation with the A gene shows gene-specific rescue for A but not B. The effect of the old macronucleus on the processing of the new macronucleus results in a pattern of non-Mendelian inheritance of both macronuclear deletions. Progeny from the wild-type exconjugant are all wild type, and progeny from the A- B- exconjugant are mutant. The features of this A- B- non-Mendelian mutant demonstrate that the regulation of macronuclear DNA processing is gene specific, and our results open the possibility that this type of regulation affects many regions of the Paramecium genome.
机译:四履带草履虫包含两种类型的核,一个二倍体生发微核和一个大的转录活性大核。大核基因组是在有性生殖过程中由微核DNA形成的。以前的研究表明,在形成新的大核期间,对A型可变表面蛋白基因的加工取决于在旧的大核中A基因的存在。尚不清楚这是控制草履虫大核基因组形成的一般特征还是A基因座的独特特征。使用微核移植,我们构建了一个具有野生型微核但具有A型和B型表面蛋白基因的大核缺失的菌株。有性繁殖后,A和B基因均未掺入新的大核中。用B基因对该菌株的大核转化在下一个大核形成后可以挽救B基因的缺失,但对A缺失没有影响。同样,用A基因转化显示出对A的基因特异性拯救,但对B却没有。老大核对新大核的加工作用导致两种大核缺失的非孟德尔遗传模式。来自野生型准分子的子代均为野生型,而来自A-B-准分子的子代均为突变体。这种A-B-非孟德尔突变体的特征表明,大核DNA加工的调控是基因特异性的,我们的结果为这种调控影响草履虫基因组的许多区域提供了可能性。

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